Additionally, the effect of YTHDC1 knockdown on CD86 and IL-6 mRNA induced by LPS/IFN-γ was abolished by the NF-κB inhibitor JSH-23. Mechanistically, YTHDC1 interacted with Beclin1 mRNA, thereby stabilizing Beclin1 mRNA and enhancing Beclin1 expression and autophagy. These effects ultimately...
(Thermo Fisher Scientific) and protease inhibitor cocktail. The clarified lysates were incubated with anti-YTHDC1 antibody or IgG overnight at 4 °C. Then 30 µl of protein A/G magnetic beads (Thermo Fisher Scientific) were added and incubated for 2 h at 4 °C. After four ...
the combination of AURKA kinase inhibitor (VX680) and AURKA nuclear localization inhibitors (JNJ or PHA) produces an additive tumor growth-suppressing effect (Fig.6e–g). Overall, the discovery of AURKA nuclear translocation inhibitors provides a novel therapeutic route ...
5D–I). qRT-PCR, western blot, and immunofluorescence assays showed that the miR-124486-5 mimic decreased the MAPK1 mRNA, t-ERK1/2 and p-ERK1/2 proteins expression, and the p-ERK1/2 level against the amount of t-ERK1/2 expression, whereas the miR-124486-5 inhibitor increased it (...
Serine protease inhibitor A3N-siRNA reduced LPS-induced inflammation of cardiac myocytes. In conclusion, the m(6)A reader YTHDC1 regulates SERPINA3N mRNA expression to mediate the levels of inflammation in SIC. Such findings add to the relationship between m(6)A reader YTHDC1 and SIC, ...
Targeting m6A modification by STM2457, a small-molecule inhibitor of METTL3, could downregulate MYCN expression and attenuate tumor proliferation. This finding provides a new alternative putative therapeutic strategy for MYCN-amplified NB.doi:10.1186/s13046-024-03040-9Yi Yang...
Metabolism properties were explored for the CYP2D6 substrate and inhibitor. The excretion property was characterized by the total clearance. Toxicity properties were investigated using AMES toxicity, hepatotoxicity, and skin sensitization. 3. Results and Discussion The structure of the YTH domain of YT...